[Katsuki, Marc]

without sample in direct reflection of BS : Mon, Dec 4, 2023 1-02-58 PM.txt

with ecoli box (beam going on top part) : Mon, Dec 4, 2023 1-08-10 PM.txt

with glass box : Mon, Dec 4, 2023 1-09-35 PM.txt

small cell dish : Mon, Dec 4, 2023 2-00-17 PM.txt

can see both azimuth and ellipticity change when applying more force with holder

small dishwith hydrogel : Mon, Dec 4, 2023 4-17-11 PM.txt

ecoli box  with hydrogel : Mon, Dec 4, 2023 4-21-00 PM.txt

glass box with hydrogel : Mon, Dec 4, 2023 4-26-43 PM.txt

More analysis will follow but it seems the container with smallest effect on birefringence is the glass box.

Used the old soleil-babinet compensator manual actuator to try to apply force to hydrogel.

pushed few milimeters and could see small changes in hydrogel birefringence but the actuator entered inside it so not really uniform force applied

added plastic plate just touching (8.xx) Mon, Dec 4, 2023 4-52-28 PM.txt

However, the plastic was not sturdy enough to apply force...

We will design a proper way to apply uniform force (surely 3D printed based on the plastic box which has same dimension has the glass one).

Despite all the tentatives to apply force, the hydrogel birefringence seemed quite constant when no more force was applied.